Regulatory

Part:BBa_K1424003:Design

Designed by: Jacob Lamb   Group: iGEM14_NTNU_Trondheim   (2014-09-25)


Kanamycin resistance for Synechocystis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

genomic DNA of kanamycin containing strain of Synechocystis sp. PCC 6803

References

Poteete et al. (2006) Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli. BioTechniques 41:261-264

Guldener U et al (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Research, Vol. 24, No. 13 2519–2524.

Gueldner U et al(2002) A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Research, Vol. 30, No. 6 e23.